Scientist (Mammalian Cell Culture)
Location: South San Francisco
Posted on: June 23, 2025
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Job Description:
Freyr is hiring We are hiring for a Mammalian Cell Culture
Scientist position with a leading pharma client at South San
Francisco, CA. The pay rate is $56/hr. Along with the hourly rate
you are eligible for the following benefits package: earned paid
sick days, matching 401k plan, and discounted health insurance.
Looking for someone with BS/MS degree experienced in mammalian cell
culture and performing transient and stable transfections for
protein expression Must haves: Prior experience in mammalian cell
culture techniques, and cell culture media preparation is a
prerequisite: single use mammalian cell culture tools employed in
bioprocess development, including but not limited to deep well
plates and shake flasks. Perform transient and stable transfections
for protein expression in mammalian cells (CHO, HEK293). Ability to
multi-task different protocols at once, handle concurrent projects
simultaneously with high attention to detail. Responsibilities: •
Maintain and propagate CHO cells in suspension culture
independently to support ongoing projects in a cell culture lab.
Perform transient and stable transfections for protein expression
in mammalian cells (CHO, HEK293). Express and batch purify a
variety of proteins (monoclonal, bispecific antibodies, VHHs, Fabs,
Fc-Fusions, etc.) using both resin and magnetic-bead
immunoprecipitation methods. Execute fed-batch experiments in a
variety of platforms: shake flasks 125ml to 5L, tube spins and
multi-well plates. Perform analytical measurements for cell growth,
metabolite measurements and HPLC analysis of titers from
supernatant. Analyze purified proteins using techniques: SDS-PAGE,
HPLC, UPLC, CESDS, Nano-drop, UV-Vis. Maintain and troubleshoot
mammalian cell culture systems. Document protein production
processes, prepare reports and communicate results ELN
documentation. . If interested, please reply at your earliest along
with a copy of your updated resume and share your availability to
discuss this opportunity further. Thank you. Aparna Ksheersagar,
Email ID: aparna.ksheersagar@freyrsolutions.com | Phone: 1
6096812406
Keywords: , Santa Rosa , Scientist (Mammalian Cell Culture), Science, Research & Development , South San Francisco, California
